MicroJournal #3
By: Bryoni Booker
Gram Staining
- Is used to tell the difference between gram +/- organisms when dealing with Microbiology
- It is a very important procedure used quite often to conclude an experiment
Gram Staining have 4 Processes:
- crystal violet, the primary stain
- iodine, the mordant
- a decolorizer made of acetone and alcohol
- safranin, the counterstain
Gram Positive VS Gram Negative
- They can be distinguished based on their cell walls
- "the way the cell takes up and retains stains" (microbugz)
Procedure:
WATCH THE GRAM-STAINING IS DONE!
Resources:
http://www.austincc.edu/microbugz/handouts/Stain%20protocols.pdf
http://www.microbiol.org/resources/monographswhite-papers/the-gram-stain/
http://www.youtube.com/watch?v=c6d7zOIP6Vo&feature=youtu.be
http://www.austincc.edu/microbugz/gram_stain.php
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